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KMID : 0380020030180020122
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 2 p.122 ~ p.126
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Purification of Lysozyme from Egg White by Multicycle Ion Exchange Chromatography
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Huh Yoon-Suk
Kim Hyung-Won
Kim In-Ho
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Abstract
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Multi-cycle chromatographic separation of Iysozyme from egg white was investigated. Multi-cycle chromatography was performed by repeated cycling(one cycle: resin equilibration, sample loading, washing, elution). Two types of cation exchange resins, Cellufine CM C-200 and Bio-rex 70, were used to determine the optimum condition for the separation of Iysozyme by multi-cycle chromatography. The resin was equilibrated in 20 mM Na-phosphate buffer(pH 7.0). Chromatograms of UV absorbance levels of every cycle were compared to confirm the eluting ability of Iysozyme in the two types of gel. Collected samples from eluting regions in every cycle were assayed by 15% SDS-PAGE.
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KEYWORD
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Chromatography, egg white, multi-cycle, lysozyme
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